Quantum yields for inactivation of tobacco mosaic virus nucleic acid by ultraviolet radiation (254 nm.).
نویسندگان
چکیده
The quantum yield, under non-photoreactivating conditions, for inactivation of tobacco mosaic virus RNA by ultraviolet radiation (254 nm.) is independent of the RNA concentration but varies appreciably with the ionic strength of the irradiation solvent, being approximately three times greater in water than in o-I M-potassium phosphate buffer. Photoreactivation of inactivated tobacco mosaic virus RNA is dependent on RNA concentration, ionic strength of solutions during irradiation, and on the light quality used for photoreactivation. These data can be interpreted as evidence that pyrimidine hydrate is a lethal lesion at least in RNA irradiated at low ionic strength.
منابع مشابه
The Ultraviolet Light and Photosensitized Inactivation of Tobacco Mosaic Virus
The quantum yield for the inactivation of tobacco mosaic virus has been determined at 253.7 mmicro and found to be 4.3 x 10(-6). The possible significance of the observed one-hit process of inactivation has been discussed in terms of the kinetics and the rupture of model substances including nucleic acid. The ultraviolet light inactivation, which proceeds independent of oxygen, occurs without c...
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عنوان ژورنال:
- The Journal of general virology
دوره 4 1 شماره
صفحات -
تاریخ انتشار 1969